ly6e antibodies for mouse  (Novus Biologicals)

Journal: PLoS Computational Biology

Article Title: Antithetic effect of interferon-α on cell-free and cell-to-cell HIV-1 infection

doi: 10.1371/journal.pcbi.1010053

Figure Lengend Snippet: a The expression levels of LY6E . Each symbol indicates the result from one replicate, and the bars with error bars indicate the averages with SEMs of three independent replicates. RPKM, reads per kilobase of exon per million mapped reads. b,c Effect of LY6E overexpression on cell-free HIV-1 infection. b Western blotting. Parental Jurkat-CCR5 cells (’Parent’), empty vector-transduced cells (Empty), and LY6E-HA-transduced cells (’LY6E-HA’) were prepared as described in Methods, and the LY6E-HA expression is detected by Western blotting. A representative result is shown. Alpha-tubulin (TUBA) is used as an internal control. c Single-round infection assay under different density conditions. The V/C at different five culture conditions (0.5, 1, 2, and 6 ml) corresponds to that shown in . Single round infection assay was performed at four different cell-virus densities. d,e Effect of LY6E KO on cell-free HIV-1 infection. d Western blotting. Parental Jurkat-CCR5 cells (’Parent’), non-target sgRNA-transduced cells (’SgNT’), and LY6E KO cells ( ’LY6E KO’) were prepared as described in Materials and Methods, and endogenous LY6E expression is detected by Western blotting. A representative result is shown. TUBA is used as an internal control. e Single-round infection assay under different density conditions. The V/C at different five culture conditions (0.5, 1, 2, 3 and 6 ml) corresponds to that shown in . Single round infection assay was performed at five different cell-virus densities. In panels c and e , each dot indicates the result from one culture, and three independent experiments were performed. The bars with error bars are the averages and SEMs of three independent experiments. Asterisks indicate statistically significant differences determined by Student’s t test ( p <0.05).

Article Snippet: LY6E was detected using a mouse anti-LY6E monoclonal antibody (4D8.6.7, Genentech), and beta-actin (ACTB) was detected using peroxidase-conjugated anti-ACTB monoclonal antibody (AC-15, Sigma).

Techniques: Expressing, Over Expression, Infection, Western Blot, Plasmid Preparation, Control, Virus